Immunoassay

prefLabel
  • immunoassay
definition
  • Any of several methods for the quantitative determination of chemical substances such as hormones, drugs, and certain proteins that utilize the highly specific binding between an antigen and an antibody.
inScheme
broader
Abstract from DBPedia
    An immunoassay (IA) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules, of different sizes and types, as long as the proper antibodies that have the required properties for the assay are developed. Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes. Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay. Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. Some immunoassays can be carried out simply by mixing the reagents and sample and making a physical measurement. Such assays are called homogeneous immunoassays, or less frequently non-separation immunoassays. The use of a calibrator is often employed in immunoassays. Calibrators are solutions that are known to contain the analyte in question, and the concentration of that analyte is generally known. Comparison of an assay's response to a real sample against the assay's response produced by the calibrators makes it possible to interpret the signal strength in terms of the presence or concentration of analyte in the sample.

    免疫学的検定(めんえきがくてきけんてい、英: immunoassay)は、抗原或いは抗体の反応を利用して血清や尿のような生物学的液体の中に含まれる物質の濃度を測定する生化学的試験である。

    (Source: http://dbpedia.org/resource/Immunoassay)