Fluorescence spectroscopy

prefLabel
  • Fluorescence Spectroscopy
definition
  • Among instrumental techniques, fluorescence spectroscopy is recognized as one of the more sensitive. In fluorescence, the intensity of the emission of the sample is measured. The reason for the high sensitivity of fluorescence techniques is that the emission signal is measured above a low background level. This is inherently more sensitive than comparing two relatively large signals as in absorption spectroscopy. The sensitivity of fluorescence techniques is as much as 1000 times more sensitive than absorption spectroscopy. Additional information available at "http://www.pti-nj.com/tech_3.html"
inScheme
broader
Abstract from DBPedia
    Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers.

    (Source: http://dbpedia.org/resource/Fluorescence_spectroscopy)